LBP Monoklonaler Antikörper

LBP Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3A7E3

Kat-Nr. : 66181-1-Ig

Synonyme

LBP

---

• 

  WB analysis of Jurkat using 66181-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66181-1-Ig (LBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66181-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66181-1-Ig (LBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66181-1-Ig bindet in WB, IF, IHC, ELISA LBP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LBP fusion protein Ag13485
• Vollständiger Name: lipopolysaccharide binding protein
• Berechnetes Molekulargewicht: 481 aa, 53 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC022256
• Gene symbol: LBP
• Gene ID (NCBI): 3929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Publikationen

Species	Application	Title
mouse,human	WB,IF	Nat Commun; Lipopolysaccharide binding protein resists hepatic oxidative stress by regulating lipid droplet homeostasis; Authors - Qilun Zhang