LBP Rekombinanter Antikörper

LBP Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230524G10

Kat-Nr. : 83427-1-RR

Synonyme

Lipopolysaccharide-binding protein, 230524G10

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  WB analysis of A431 using 83427-1-RR

  A431 cells were subjected to SDS PAGE followed by western blot with 83427-1-RR (LBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83427-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83427-1-RR against Human LBP were performed. The affinity constant is 27.1 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83427-1-RR bindet in WB, ELISA LBP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: lipopolysaccharide binding protein
• Berechnetes Molekulargewicht: 53KD
• Beobachtetes Molekulargewicht: 63-67 kDa
• GenBank-Zugangsnummer: NM_004139.4
• Gene symbol: LBP
• Gene ID (NCBI): 3929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)