LBR Rekombinanter Antikörper

LBR Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241864H1

Kat-Nr. : 84522-6-PBS

Synonyme

Delta(14)-sterol reductase LBR, C14SR, C-14 sterol reductase, 3-beta-hydroxysterol Delta, 241864H1

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  WB analysis using 84522-6-RR (same clone as 84522-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84522-6-RR (LBR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84522-6-RR (same clone as 84522-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84522-6-RR (same clone as 84522-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug LBR Recombinant antibody (84522-6-RR, Clone:241864H1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84522-6-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84522-6-PBS bindet in WB, FC (Intra), Indirect ELISA LBR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LBR fusion protein Ag3088
• Vollständiger Name: lamin B receptor
• Berechnetes Molekulargewicht: 615 aa, 71 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC020079
• Gene symbol: LBR
• Gene ID (NCBI): 3930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamin-B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that contains a hydrophilic N-terminal end protruding into the nucleoplasm, eight hydrophobic segments that span the membrane and a short, nucleoplasmic C-terminal tail (PMID: 28858257). LBR anchors the lamina and the heterochromatin to the inner nuclear membrane (PMID: 10828963; 28858257). It is also essential for cholesterol synthesis (PMID: 27336722). Mutations of the LBR gene have been associated with autosomal recessive HEM/Greenberg skeletal dysplasia and Pelger-Huët anomaly and Greenberg skeletal dysplasia (PMID: 12618959; 12490533). The calculated molecular mass of LBR is 71 kDa, which is larger than the apparent molecular mass of 58 kDa, probably due to the aberrant migration of membrane proteins subjected to SDS-PAGE (PMID: 2847165; 2170422).