LDB1 Monoklonaler Antikörper

LDB1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F11C3

Kat-Nr. : 68721-1-Ig

Synonyme

CLIM 2, CLIM2, hLdb1, LDB 1, LDB1, LIM domain binding 1, LIM domain binding protein 1, NLI, Nuclear LIM interactor

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  WB analysis using 68721-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68721-1-Ig (LDB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 68721-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68721-1-Ig (LDB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 68721-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68721-1-Ig (LDB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68721-1-Ig bindet in WB, ELISA LDB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LDB1 fusion protein Ag34563
• Vollständiger Name: LIM domain binding 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC000482
• Gene symbol: LDB1
• Gene ID (NCBI): 8861
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIM domain-binding protein 1 (LDB1), also known as Carboxyl-terminal LIM domain-binding protein 2 (CLIM2), binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. LDB1 may regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. It plays a role in the development of interneurons and motor neurons in cooperation with LHX3 and ISL1. LDB1 acts synergistically with LHX1/LIM1 in axis formation and activation of gene expression. It also acts with LMO2 in the regulation of red blood cell development, maintaining erythroid precursors in an immature state. LDB1 strongly interacts with the LIM2 domain of LMX1A to form homodimer. LDB1 protein is expressed in a wide range of adult tissues including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.