LDHA-Specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

LDHA-Specific Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240885F4

Kat-Nr. : 84198-1-PBS

Synonyme

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

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  WB analysis of MDA-MB-231 using 84198-1-RR (same clone as 84198-1-PBS)

  WB result of LDHA-Specific antibody (84198-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84198-1-RR (same clone as 84198-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-RR, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84198-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84198-1-PBS

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LDHA-Specific antibody (84198-1-PBS, Clone: 240885F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84198-1-PBS bindet in WB, IF/ICC, Indirect ELISA LDHA-Specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lactate dehydrogenase A
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: NM_005566
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.