LDHA-Specific Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

LDHA-Specific Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240885A9

Kat-Nr. : 84198-2-PBS

Synonyme

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

---

• 

  WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84198-2-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MDA-MB-231 using 84198-2-RR (same clone as 84198-2-PBS)

  WB result of LDHA-Specific antibody (84198-2-RR; 1:3000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-2-RR, Clone:240885A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84198-2-PBS bindet in WB, FC (Intra), Indirect ELISA LDHA-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lactate dehydrogenase A
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_005566
• Gene symbol: LDHA
• Gene ID (NCBI): 3939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.