LDHD Polyklonaler Antikörper

LDHD Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14398-1-PBS

Synonyme

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  WB analysis using 14398-1-AP (same clone as 14398-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human osteosarcoma using 14398-1-AP (same clone as 14398-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14398-1-AP (LDHD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14398-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LDHD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LDHD fusion protein Ag5897
• Vollständiger Name: lactate dehydrogenase D
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 45-54 kDa
• GenBank-Zugangsnummer: BC047902
• Gene symbol: LDHD
• Gene ID (NCBI): 197257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.