LDLR Polyklonaler Antikörper

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• KD/KO Validated

LDLR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10785-1-PBS

Synonyme

FH, FHC, LDL receptor

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  WB analysis using 10785-1-AP (same clone as 10785-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 10785-1-AP (same clone as 10785-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 10785-1-AP (same clone as 10785-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10785-1-AP (same clone as 10785-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10785-1-AP (LDLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10785-1-AP (same clone as 10785-1-PBS)

  IP result of anti-LDLR (IP:10785-1-AP, 5ug; Detection:10785-1-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10785-1-AP (LDLR Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10785-1-AP (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 10785-1-AP (same clone as 10785-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10785-1-AP (LDLR antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 10785-1-AP (same clone as 10785-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (10785-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red). This data was developed using the same antibody clone with 10785-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10785-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA LDLR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LDLR fusion protein Ag1236
• Vollständiger Name: low density lipoprotein receptor
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC014514
• Gene symbol: LDLR
• Gene ID (NCBI): 3949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.