LDLR Monoklonaler Antikörper

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• KD/KO Validated

LDLR Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2A3

Kat-Nr. : 66414-1-PBS

Synonyme

1A2A3, FH, FHC, LDL receptor

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  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  WB result of LDLR antibody (66414-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LDLR transfected HeLa cells. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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  WB analysis using 66414-1-Ig (same clone as 66414-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66414-1-Ig (same clone as 66414-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66414-1-Ig (same clone as 66414-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66414-1-Ig (same clone as 66414-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66414-1-Ig (LDLR Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66414-1-Ig (same clone as 66414-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66414-1-Ig (LDLR Antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66414-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66414-1-PBS bindet in WB, IHC, ELISA LDLR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LDLR fusion protein Ag1236
• Vollständiger Name: low density lipoprotein receptor
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC014514
• Gene symbol: LDLR
• Gene ID (NCBI): 3949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.