LDLR Rekombinanter Antikörper

LDLR Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1O11

Rezensionen (1)

Kat-Nr. : 82724-1-RR

Synonyme

1O11, FH, FHC, LDL receptor

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  WB analysis using 82724-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 82724-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82724-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 82724-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 82724-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody (82724-1-RR, Clone: 1O11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82724-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82724-1-RR against Human LDLR were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82724-1-RR bindet in WB, IHC, IF/ICC, ELISA LDLR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LDLR fusion protein Ag1236
• Vollständiger Name: low density lipoprotein receptor
• Berechnetes Molekulargewicht: 95 kDa
• Beobachtetes Molekulargewicht: 100-160 kDa
• GenBank-Zugangsnummer: BC014514
• Gene symbol: LDLR
• Gene ID (NCBI): 3949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ioana (Verified Customer) (08-28-2025)

It worked very well for immunofluorescence, allowing colocalization with early endosomal markets.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HeLaM