LEF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LEF1 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

38

Kat-Nr. : 14972-1-AP

Synonyme

LEF 1, LEF1, TCF1 alpha, TCF1ALPHA

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 14972-1-AP

  WB result of LEF1 antibody (14972-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LEF1 transfected Jurkat cells.

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  WB analysis using 14972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14972-1-AP (LEF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14972-1-AP

  WB result of LEF1 antibody (14972-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LEF1 transfected COLO 320 cells.

• 

  WB analysis using 14972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14972-1-AP (LEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 14972-1-AP

  IP Result of anti-LEF1 (IP:14972-1-AP, 4ug; Detection:14972-1-AP 1:300) with SW 1990 cells lysate 2400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, NCCIT-Zellen
• Erfolgreiche IP: SW 1990-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IHC: See 5 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

14972-1-AP bindet in WB, IP, IHC, IF, ELISA LEF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LEF1 fusion protein Ag6882
• Vollständiger Name: lymphoid enhancer-binding factor 1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 65 kDa
• GenBank-Zugangsnummer: BC050632
• Gene symbol: LEF1
• Gene ID (NCBI): 51176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory protein share homology with high mobility group protein-1, and it's a nuclear protein exprssed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway and hair cell differentiation and follicle morphogenesis. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established lasting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivopromoted self-limited airway regeneration in the absence of injury. Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. (https://doi.org/10.1016/j.stem.2018.03.017) The phosphorylation may affects LEF1 protein's theoretical molecular weight when tested.40-70 kD bands have also been reported (PMID: 22261717; 17063141 ).

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
mouse	WB,IF	J Allergy Clin Immunol; Dedicator of cytokinesis protein 2 couples with lymphoid enhancer-binding factor 1 to regulate expression of CD21 and B-cell differentiation.; Authors - Yukai Jing
mouse	WB	Cell Death Differ; MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.; Authors - Lifang Hu
human	WB	Clin Cancer Res; microRNA-221 Enhances MYCN via Targeting Nemo-like Kinase and Functions as an Oncogene Related to Poor Prognosis in Neuroblastoma.; Authors - Xiao-Yan He
human	WB	Cancer Lett; TIMELESS confers cisplatin resistance in nasopharyngeal carcinoma by activating the Wnt/β-catenin signaling pathway and promoting the epithelial mesenchymal transition.; Authors - Sai-Lan Liu ; KD Validated
mouse		Cell Death Dis; Inhibition of phosphodiesterase 5 reduces bone mass by suppression of canonical Wnt signaling.; Authors - Y Gong