LEF1 Polyklonaler Antikörper

LEF1 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 28540-1-AP

Synonyme

DKFZp586H0919, LEF 1, LEF1, TCF1 alpha, TCF1ALPHA

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Galerie der Validierungsdaten

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  WB analysis using 28540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28540-1-AP (LEF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28540-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28540-1-AP (LEF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 28540-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 28540-1-AP (LEF1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Jurkat-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

28540-1-AP bindet in WB, IF, ELISA LEF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LEF1 fusion protein Ag29841
• Vollständiger Name: lymphoid enhancer-binding factor 1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC050632
• Gene symbol: LEF1
• Gene ID (NCBI): 51176
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphoid enhancer-binding factor 1(LEF1) belongs to a family of regulatory protein share homology with high mobility group protein-1, and it's a nuclear protein exprssed in pre-B and T cells. LEF1 has a role in the Wnt signaling pathway and hair cell differentiation and follicle morphogenesis. LEF1 exists as seven isoforms and we detects three isoforms with MW 44 kDa, 36 kDa and 23 kDa. Together with CTNNB1 and EP300, LEF1 activates transcription of target genes. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. MECs can give rise to seven cell types of the SAE and SMGs following severe airway injury. MECs progressively adopted a basal cell phenotype on the SAE and established lasting progenitors capable of further regeneration following reinjury. MECs activate Wnt-regulated transcription factors (Lef-1/TCF7) following injury and Lef-1 induction in cultured MECs promoted transition to a basal cell phenotype. Surprisingly, dose-dependent MEC conditional activation of Lef-1in vivopromoted self-limited airway regeneration in the absence of injury. Thus, modulating the Lef-1 transcriptional program in MEC-derived progenitors may have regenerative medicine applications for lung diseases. (https://doi.org/10.1016/j.stem.2018.03.017) The phosphorylation may affects LEF1 protein's theoretical molecular weight when tested.40-70 kD bands have also been reported (PMID:22261717;17063141 ).

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; Long noncoding RNA LINC01606 protects colon cancer cells from ferroptotic cell death and promotes stemness by SCD1-Wnt/β-catenin-TFE3 feedback loop signalling.; Authors - Yajun Luo
rat	WB	Int J Neurosci; The dynamic expression of canonical Wnt/β-catenin signalling pathway in the pathologic process of experimental autoimmune neuritis.; Authors - Yin Liu
human	WB	Cancer Med; Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling; Authors - Xiong Shu
		Clin Exp Immunol; Dissecting T cell heterogeneity in esophageal squamous-cell carcinoma reveal the potential role of LAIR2 in anti-tumor immunity; Authors - Ping Wang