LEPREL2 Monoklonaler Antikörper

LEPREL2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6F3

Kat-Nr. : 68299-1-PBS

Synonyme

GRCB, HSU47926, leprecan like 2, Leprecan like protein 2, LEPREL2, P3H3, Prolyl 3 hydroxylase 3, Protein B

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  WB analysis of HeLa using 68299-1-Ig (same clone as 68299-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 68299-1-Ig (same clone as 68299-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 68299-1-Ig (LEPREL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 68299-1-Ig (same clone as 68299-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 68299-1-Ig (LEPREL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68299-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68299-1-PBS bindet in WB, IHC, Indirect ELISA LEPREL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LEPREL2 fusion protein Ag8850
• Vollständiger Name: leprecan-like 2
• Berechnetes Molekulargewicht: 736 aa, 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC017217
• Gene symbol: LEPREL2
• Gene ID (NCBI): 10536
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P3H3 (prolyl 3-hydroxylase 3, also known as LEPREL2) is a member of the leprecan family of proteins, which also include P3H1, P3H2, CRTAP and SC56. Collagen prolyl hydroxylases are required for proper collagen biosynthesis, folding, and assembly. P3H3 is thought to reside in the endoplasmic reticulum and has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V.