LETM1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LETM1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 16024-1-AP

Synonyme

LETM1

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Galerie der Validierungsdaten

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  WB analysis using 16024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16024-1-AP (LETM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 16024-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16024-1-AP (LETM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16024-1-AP (LETM1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HeLa using 16024-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LETM1 antibody (16024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

16024-1-AP bindet in WB, IHC, IF, CoIP, ELISA LETM1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LETM1 fusion protein Ag8861
• Vollständiger Name: leucine zipper-EF-hand containing transmembrane protein 1
• Berechnetes Molekulargewicht: 739 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC021208
• Gene symbol: LETM1
• Gene ID (NCBI): 3954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LETM1 is a mitochondrial inner membrane protein. It functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. It served as an anchor protein for complex formation between mitochondria and ribosome, and regulated mitochondrial biogenesis. It was also identified inWolf-Hirschhorn syndrome, and was deleted in nearly all patients with the syndrome. A recent study determined that adenovirus-mediated LETM1 may provide a useful target for designing lung tumor prevention and treatment.

Publikationen

Species	Application	Title
mouse	WB	Nature; The mitochondrial Na(+)/Ca(2+) exchanger is essential for Ca(2+) homeostasis and viability.; Authors - Timothy S Luongo
human	WB	Sci Adv; A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance.; Authors - Giulia Rossetti
mouse	WB	Nat Commun; Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.; Authors - Tiffany Chern
mouse	WB	Proc Natl Acad Sci U S A; Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.; Authors - Jiang Dawei D ; KO Validated
mouse	WB	Cell Rep; The Mitochondrial Calcium Uniporter Matches Energetic Supply with Cardiac Workload during Stress and Modulates Permeability Transition.; Authors - Timothy S Luongo
human	WB	Int J Mol Sci; Sunitinib and Pterostilbene Combination Treatment Exerts Antitumor Effects in Gastric Cancer via Suppression of PDZD8.; Authors - Yudai Hojo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SITING (Verified Customer) (08-31-2020)

easy to expose

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mice heart

LETM1 Antibody Western Blot validation (1:1000 dilution) in mice heart (Cat no:16024-1-AP)