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LGALS3BP Polyklonaler Antikörper

LGALS3BP Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23307-1-AP

Synonyme

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of HepG2 using 23307-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    WB analysis of mouse brain using 23307-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human breast cancer using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using LGALS3BP Polyclonal antibody (23307-1-AP)

    IHC staining of human skin using 23307-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LGALS3BP Polyclonal antibody (23307-1-AP)

    IF Staining of HepG2 using 23307-1-AP

    Immunofluorescent analysis of HepG2 cells using 23307-1-AP (LGALS3BP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, Maushirngewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Hautgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

23307-1-AP bindet in WB, IHC, IF,ELISA LGALS3BP und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LGALS3BP fusion protein Ag19700
Vollständiger Name lectin, galactoside-binding, soluble, 3 binding protein
Berechnetes Molekulargewicht 585 aa, 65 kDa
Beobachtetes Molekulargewicht 65-70 kDa
GenBank-ZugangsnummerBC002403
Gene symbol LGALS3BP
Gene ID (NCBI) 3959
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).

Protokolle

Produktspezifische Protokolle
WB protocol for LGALS3BP antibody 23307-1-APProtokoll herunterladen
IHC protocol for LGALS3BP antibody 23307-1-APProtokoll herunterladen
IF protocol for LGALS3BP antibody 23307-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen