LGALS3BP Polyklonaler Antikörper

LGALS3BP Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23307-1-PBS

Synonyme

90K, BTBD17B, Galectin 3 binding protein, Galectin-3-binding protein, Lectin galactoside-binding soluble 3-binding protein

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  WB analysis of HepG2 using 23307-1-AP (same clone as 23307-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 23307-1-AP (same clone as 23307-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23307-1-AP (LGALS3BP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 23307-1-AP (same clone as 23307-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 23307-1-AP (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 23307-1-AP (same clone as 23307-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LGALS3BP antibody (23307-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 23307-1-AP (same clone as 23307-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LGALS3BP antibody (23307-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 23307-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23307-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LGALS3BP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGALS3BP fusion protein Ag19700
• Vollständiger Name: lectin, galactoside-binding, soluble, 3 binding protein
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC002403
• Gene symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ).