LGALS3BP Monoklonaler Antikörper

LGALS3BP Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2D8E11

Kat-Nr. : 60066-1-Ig

Synonyme

90K, BTBD17B, Galectin 3 binding protein, LGALS3BP, M2BP, Mac 2 binding protein, MAC 2 BP, MAC2BP, Tumor associated antigen 90K

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Galerie der Validierungsdaten

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  WB analysis of human blood using 60066-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 60066-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 60066-1-Ig

  IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:1000) with human plasma lysate 4000ug.

• 

  IP experiment of human plasma using 60066-1-Ig

  IP Result of anti-LGALS3BP (IP:60066-1-Ig, 5ug; Detection:60066-1-Ig 1:300) with human plasma lysate 4000ug.

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  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 60066-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 60066-1-Ig (LGALS3BP Antibody) at dilution of 1:50.

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  IF Staining of A431 using 60066-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LGALS3BP antibody (60066-1-Ig, Clone: 2D8E11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Blutgewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hautkrebsgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

60066-1-Ig bindet in WB, IP, IHC, IF, ELISA LGALS3BP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LGALS3BP fusion protein Ag0294
• Vollständiger Name: lectin, galactoside-binding, soluble, 3 binding protein
• Berechnetes Molekulargewicht: 585 aa, 65 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC002403
• Gene symbol: LGALS3BP
• Gene ID (NCBI): 3959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-3-binding protein (LGALS3BP, also known as 90K or Mac-2 BP) is a secreted glycoprotein which binds galectin-3, galectin-1, beta1 integrins, collagens and fibronectin (PMID: 8034587; 8024581; 11146440; 9501082). It has been implicated in tumor metastatic processes, as well as in other cell adhesion and immune functions. Levels of LGALS3BP have been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV) (PMID: 7698018). Western analysis suggests that LGALS3BP is found in breast milk, semen, saliva, urine, and tears, in addition to serum (PMID: 8390986 ). LGALS3BP has a predicted mass of ∼65 kDa. It is heavily glycosylated and shows an apparent molecular weight of 90 kDa on SDS-PAGE.

Publikationen

Species	Application	Title
mouse	WB	PLoS Pathog; Inducible LGALS3BP/90K activates antiviral innate immune responses by targeting TRAF6 and TRAF3 complex.; Authors - Gang Xu
human	WB	Invest Ophthalmol Vis Sci; LGALS3BP in Microglia Promotes Retinal Angiogenesis Through PI3K/AKT Pathway During Hypoxia.; Authors - Chenyang Zhao
human	WB	Proteomics; Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles; Authors - Yaojie Wang
human	WB	bioRxiv; Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2; Authors - Aswini Panigrahi

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