Galectin-4 Polyklonaler Antikörper

Galectin-4 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 27552-1-AP

Synonyme

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

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Galerie der Validierungsdaten

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  WB analysis using 27552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27552-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 27552-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 27552-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 27552-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27552-1-AP (LGALS4,GAL4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 27552-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using Galectin-4 antibody (27552-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Kolongewebe, Maus-Dünndarmgewebe, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

27552-1-AP bindet in WB, IHC, IF, ELISA Galectin-4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-4 fusion protein Ag26130
• Vollständiger Name: lectin, galactoside-binding, soluble, 4
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC034750
• Gene symbol: LGALS4
• Gene ID (NCBI): 3960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

Publikationen

Species	Application	Title
mouse	WB	Front Cell Dev Biol; Single-Cell Transcriptomics Reveals Splicing Features of Adult Neural Stem Cells in the Subventricular Zone.; Authors - Yanlu Wang
human	WB	Cell Adh Migr; SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.; Authors - Yusuke Mizutani