LGALS9/Galectin-9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LGALS9/Galectin-9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 17938-1-AP

Synonyme

Galectin 9, LGALS9, Ecalectin, Gal 9, Gal-9

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  WB analysis using 17938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17938-1-AP (LGALS9/Galectin-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17938-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17938-1-AP (Galectin-9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-937 using 17938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-937 cells using LGALS9/Galectin-9 antibody (17938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, L02-Zellen, NIH/3T3-Zellen, THP-1-Zellen, U-937-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

17938-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LGALS9/Galectin-9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGALS9/Galectin-9 fusion protein Ag12209
• Vollständiger Name: lectin, galactoside-binding, soluble, 9
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa
• GenBank-Zugangsnummer: BC110340
• Gene symbol: Galectin-9
• Gene ID (NCBI): 3965
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Publikationen

Species	Application	Title
human	IHC	Cell Rep Med; Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma; Authors - Junju Chen
	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB,IHC	Front Immunol; Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.; Authors - Guanzhang Li
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Stem Cell Res Ther; Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.; Authors - Junyu Fan