LGMN Polyklonaler Antikörper

LGMN Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15095-1-PBS

Synonyme

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  WB analysis using 15095-1-AP (same clone as 15095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15095-1-AP (LGMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 15095-1-AP (same clone as 15095-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15095-1-AP (LGMN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15095-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15095-1-PBS bindet in WB, IHC, Indirect ELISA LGMN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGMN fusion protein Ag7170
• Vollständiger Name: legumain
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC003061
• Gene symbol: LGMN
• Gene ID (NCBI): 5641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).