LGMN Monoklonaler Antikörper

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LGMN Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F2B12

Kat-Nr. : 67017-1-PBS

Synonyme

PRSC1, legumain, EC:3.4.22.34, 1F2B12

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  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67017-1-Ig (same clone as 67017-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 67017-1-Ig (same clone as 67017-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67017-1-Ig (same clone as 67017-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67017-1-Ig (same clone as 67017-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 67017-1-Ig (LGMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67017-1-Ig (LGMN antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67017-1-Ig (same clone as 67017-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using LGMN antibody (67017-1-Ig, Clone: 1F2B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67017-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67017-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA LGMN und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LGMN fusion protein Ag7218
• Vollständiger Name: legumain
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC003061
• Gene symbol: LGMN
• Gene ID (NCBI): 5641
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).