LGTN Polyklonaler Antikörper

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• KD/KO Validated

LGTN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 12840-1-AP

Synonyme

ligatin, Hepatocellular carcinoma-associated antigen 56, HCA56, EIF2D

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  WB analysis using 12840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12840-1-AP (LGTN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12840-1-AP

  IP result of anti-LGTN (IP:12840-1-AP, 4ug; Detection:12840-1-AP 1:2000) with HepG2 cells lysate 1320 ug.

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  IHC staining of human stomach using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 12840-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12840-1-AP (LGTN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12840-1-AP

  Immunofluorescent analysis of HepG2 cells, using LGTN antibody 12840-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, PC-3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

12840-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA LGTN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LGTN fusion protein Ag3579
• Vollständiger Name: ligatin
• Berechnetes Molekulargewicht: 584 aa, 65 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC001585
• Gene symbol: LGTN
• Gene ID (NCBI): 1939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Science; Leucine-tRNA initiates at CUG start codons for protein synthesis and presentation by MHC class I.; Authors - Shelley R Starck ; KD Validated
human	IF	Mol Cell; Spatiotemporal Proteomic Analysis of Stress Granule Disassembly Using APEX Reveals Regulation by SUMOylation and Links to ALS Pathogenesis.; Authors - Hagai Marmor-Kollet
rat	WB	Genes Dev; Activities of Ligatin and MCT-1/DENR in eukaryotic translation initiation and ribosomal recycling.; Authors - Maxim A Skabkin
human,mouse	WB	Nat Commun; A C. elegans model of C9orf72-associated ALS/FTD uncovers a conserved role for eIF2D in RAN translation.; Authors - Yoshifumi Sonobe ; KD Validated
human	WB	Nucleic Acids Res; Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.; Authors - Kazuya Ichihara ; KO Validated
human	IHC	Proc Natl Acad Sci U S A; Local translation in nuclear condensate amyloid bodies.; Authors - Phaedra R Theodoridis