LHCGR Polyklonaler Antikörper

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• KD/KO Validated

LHCGR Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Kat-Nr. : 19968-1-AP

Synonyme

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing Hormone Receptor

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Galerie der Validierungsdaten

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  WB analysis using 19968-1-AP

  Unboiled mouse testis tissue was subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19968-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19968-1-AP

  IP Result of anti-LHCGR (IP:19968-1-AP, 4ug; Detection:19968-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of mouse ovary using 19968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (19968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 19968-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LHCGR (19968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochtes Maushodengewebe, Maus-Eierstockgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF: Maus-Eierstockgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 3 publications below

Produktinformation

19968-1-AP bindet in WB, IP, IHC, IF, FC, ELISA LHCGR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: LHCGR
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_000233
• Gene symbol: LHCGR
• Gene ID (NCBI): 3973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publikationen

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
human	WB	Am J Pathol; Amyloid Precursor Protein Overexpression in Down Syndrome Trophoblast Reduces Cell Invasiveness and Interferes with Syncytialization.; Authors - Oscar Gee Wan Wong
mouse	WB	PLoS One; The RNA-Binding Protein, ZFP36L2, Influences Ovulation and Oocyte Maturation.; Authors - Christopher B Ball
mouse	WB	Chemosphere; PMID: 28081510; Authors - Xiaoyang Wang
mouse	WB,IHC	Front Endocrinol (Lausanne); Downregulation of LHCGR Attenuates COX-2 Expression and Induces Luteinized Unruptured Follicle Syndrome in Endometriosis.; Authors - Ting Geng

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