LHCGR Polyklonaler Antikörper

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• KD/KO Validated

LHCGR Polyklonal Antikörper für WB, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(33)

Kat-Nr. : 19968-1-AP

Synonyme

LHR, LCGR, LGR2, LH R, LH receptor

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  WB analysis using 19968-1-AP

  Unboiled mouse testis tissue was subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19968-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19968-1-AP

  IP result of anti-LHCGR (IP:19968-1-AP, 4ug; Detection:19968-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of mouse ovary using 19968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (19968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochtes Maushodengewebe, Maus-Eierstockgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF-P: Maus-Eierstockgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 33 publications below
• IHC: See 5 publications below

Produktinformation

19968-1-AP bindet in WB, IHC, IF-P, IP, ELISA LHCGR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: LHCGR
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_000233
• Gene symbol: LHCGR
• Gene ID (NCBI): 3973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs). It plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in the LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publikationen

Species	Application	Title
rat	WB	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
mouse	WB	Environ Pollut; Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway; Authors - Haibo Jin
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
pig	WB	Biol Res; Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model; Authors - Muhammad Ameen Jamal
human	WB	Reprod Biol Endocrinol; miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4; Authors - Wenhan Ju