LHPP Polyklonaler Antikörper

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• KD/KO Validated

LHPP Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 15759-1-AP

Synonyme

hLHPP, LHPP

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Galerie der Validierungsdaten

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  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15759-1-AP

  WB result of LHPP antibody (15759-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHPP transfected A549 cells.

• 

  WB analysis of mouse liver using 15759-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15759-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15759-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15759-1-AP

  Immunofluorescent analysis of Hela cells, using LHPP antibody 15759-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A431-Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Lebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

15759-1-AP bindet in WB, IHC, IF, ELISA LHPP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LHPP fusion protein Ag8375
• Vollständiger Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Berechnetes Molekulargewicht: 23 kDa, 29 kDa
• Beobachtetes Molekulargewicht: 23-32 kDa
• GenBank-Zugangsnummer: BC007324
• Gene symbol: LHPP
• Gene ID (NCBI): 64077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB	Biochim Biophys Acta Mol Basis Dis; Correlated with better prognosis, CSTA inhibits metastasis of nasopharyngeal carcinoma cells via suppressing AKT signaling through promoting METTL3 degradation; Authors - Zhi-Jie Liu
human	WB	Aging (Albany NY); The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.; Authors - Xuhui Yang ; KD Validated
human	WB	Cell Cycle; LHPP inhibits hepatocellular carcinoma cell growth and metastasis.; Authors - Lijuan Liao
human	WB,IHC,IF	Oncol Rep; Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway.; Authors - Bin Hou
human	WB	J Physiol Biochem; Tumor suppressor LHPP suppresses cell proliferation and epithelial-mesenchymal transition in hepatocellular carcinoma cell lines.; Authors - Lunkun Ma ; KD Validated