LIAS Monoklonaler Antikörper

LIAS Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3B5G7

Kat-Nr. : 67298-1-PBS

Synonyme

3B5G7, EC:2.8.1.8, HUSSY 01, HUSSY-01, LAS

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  WB analysis of LNCaP using 67298-1-Ig (same clone as 67298-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67298-1-Ig (same clone as 67298-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67298-1-Ig (LIAS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67298-1-Ig (same clone as 67298-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67298-1-Ig (LIAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67298-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67298-1-PBS bindet in WB, IHC, ELISA LIAS und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LIAS fusion protein Ag27286
• Vollständiger Name: lipoic acid synthetase
• Berechnetes Molekulargewicht: 372 aa, 42 kDa
• Beobachtetes Molekulargewicht: 34-42 kDa
• GenBank-Zugangsnummer: BC023635
• Gene symbol: LIAS
• Gene ID (NCBI): 11019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIAS(lipoyl synthase, mitochondrial) is also named as LAS and belongs to the radical SAM superfamily and lipoyl synthase family. It produces alpha-lipoic acid, an antioxidant and an essential cofactor in alpha-ketoacid dehydrogenase complexes, which participate in glucose oxidation and ATP generation(PMID:22021711). The deduced 373-amino acid protein has a calculated molecular mass of about 42 kD. The N-terminal 26 amino acids encode a potential mitochondrial targeting presequence that, upon removal, would result in a deduced mature protein of 347 amino acids with a molecular mass of about 39 kD(PMID:11389890). Defects in LIAS are a cause of pyruvate dehydrogenase lipoic acid synthetase deficiency (PDHLD).