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LIF Polyklonaler Antikörper

LIF Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26757-1-PBS

Synonyme

Leukemia inhibitory factor, Differentiation-stimulating factor, Melanoma-derived LPL inhibitor

Filter:
  • Western Blot (WB) analysis of various lysates using LIF Polyclonal antibody (26757-1-AP)

    WB analysis using 26757-1-AP (same clone as 26757-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using LIF Polyclonal antibody (26757-1-AP)

    WB analysis of MCF-7 using 26757-1-AP (same clone as 26757-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 26757-1-AP (LIF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of human lung cancer using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IHC staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 26757-1-AP (LIF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse embryo tissue using LIF Polyclonal antibody (26757-1-AP)

    IF Staining of mouse embryo using 26757-1-AP (same clone as 26757-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using LIF antibody (26757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LIF Polyclonal antibody (26757-1-AP)

    IF Staining of A431 using 26757-1-AP (same clone as 26757-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LIF antibody (26757-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 26757-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

26757-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA LIF und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LIF fusion protein Ag25076
Vollständiger Name leukemia inhibitory factor (cholinergic differentiation factor)
Berechnetes Molekulargewicht 202 aa, 22 kDa
Beobachtetes Molekulargewicht 22 kDa, 32 kDa
GenBank-ZugangsnummerBC093733
Gene symbol LIF
Gene ID (NCBI) 3976
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein with variable molecular weight (38-67 kDa) due to differential glycosylation. (PMID: 8262236, PMID: 35204717)