DNA Ligase I Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNA Ligase I Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 18051-1-AP

Synonyme

DNA ligase 1, DNA Ligase I, LIG1, ligase I, DNA, ATP dependent

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Galerie der Validierungsdaten

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  WB analysis using 18051-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18051-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18051-1-AP (DNA Ligase I antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 18051-1-AP

  IP Result of anti-DNA Ligase I (IP:18051-1-AP, 4ug; Detection:18051-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human spleen using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18051-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18051-1-AP (DNA Ligase I antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

18051-1-AP bindet in WB, IP, IHC, ELISA DNA Ligase I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNA Ligase I fusion protein Ag12508
• Vollständiger Name: ligase I, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 919 aa, 102 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC108318
• Gene symbol: LIG1
• Gene ID (NCBI): 3978
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 18051-1-AP is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Publikationen

Species	Application	Title
mouse	WB	Nature; eccDNAs are apoptotic products with high innate immunostimulatory activity; Authors - Yuangao Wang
human	WB,IHC	Mol Cancer; hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma; Authors - Lei Xu
mouse	WB	Nat Commun; Cas9-induced large deletions and small indels are controlled in a convergent fashion.; Authors - Michael Kosicki
human	WB,IP	J Clin Invest; Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.; Authors - Patrick Maffucci ; KO Validated
rat	WB	Proc Natl Acad Sci U S A; Redundant function of DNA ligase 1 and 3 in alternative end-joining during immunoglobulin class switch recombination.; Authors - Shahnaz Masani ; KO Validated
mouse	WB	Proc Natl Acad Sci U S A; Ligase I and ligase III mediate the DNA double-strand break ligation in alternative end-joining.; Authors - Guangqing Lu ; KO Validated