DNA ligase III/LIG3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

DNA ligase III/LIG3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Rezensionen (1)

Kat-Nr. : 26583-1-AP

Synonyme

LIG3, ligase III, DNA, ATP dependent, LIG2, EC:6.5.1.1, DNA ligase III

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• 

  WB analysis using 26583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 26583-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26583-1-AP (DNA ligase III/LIG3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 26583-1-AP

  IP result of anti-DNA ligase III/LIG3 (IP:26583-1-AP, 4ug; Detection:26583-1-AP 1:300) with HEK-293T cells lysate 4000ug.

• 

  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 26583-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26583-1-AP (DNA ligase III/LIG3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26583-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNA ligase III/LIG3 antibody (26583-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Produktinformation

26583-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA DNA ligase III/LIG3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DNA ligase III/LIG3 fusion protein Ag24998
• Vollständiger Name: ligase III, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 100-110 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC068005
• Gene symbol: LIG3
• Gene ID (NCBI): 3980
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA ligase III(DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

Publikationen

Species	Application	Title
human	IF	Brain; Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy.; Authors - Elena Bonora
pig	WB	Int J Biochem Cell Biol; Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors.; Authors - Guoling Li
human	WB	bioRxiv; Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy; Authors - Zhihua Kang
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona
mouse	WB	J Alzheimers Dis Rep; Genome-Wide Mendelian Randomization Identifies Ferroptosis-Related Drug Targets for Alzheimer's Disease; Authors - Ying Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MANOHAR (Verified Customer) (12-11-2019)

No nonspecific binding

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T