DNA ligase III/LIG3 Monoklonaler Antikörper

DNA ligase III/LIG3 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10B3

Kat-Nr. : 68810-1-PBS

Synonyme

LIG3, 1B10B3, DNA ligase 3, DNA ligase III, EC:6.5.1.1

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  WB analysis using 68810-1-Ig (same clone as 68810-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68810-1-Ig (DNA ligase III/LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68810-1-Ig (same clone as 68810-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68810-1-Ig (LIG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68810-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50683-2

  Cytometric bead array standard curve of MP50683-2, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-3-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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  Cytometric bead array standard curve of MP50683-3

  Cytometric bead array standard curve of MP50683-3, DNA ligase III/LIG3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68810-1-PBS. Detection antibody: 68810-2-PBS. Standard:Ag31484. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68810-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA DNA ligase III/LIG3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: DNA ligase III/LIG3 fusion protein Ag31484
• Vollständiger Name: ligase III, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 100-110 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC068005
• Gene symbol: LIG3
• Gene ID (NCBI): 3980
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.