DNA ligase III/LIG3 Rekombinanter Antikörper

DNA ligase III/LIG3 Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242870E11

Kat-Nr. : 85409-1-PBS

Synonyme

LIG3, DNA ligase 3, DNA ligase III, EC:6.5.1.1, LIG2

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  Sandwich ELISA standard curve of MP01953-3

  Sandwich ELISA standard curve of MP01953-3, Human DNA ligase III/LIG3 Recombinant Matched Antibody Pair - PBS only. 85409-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24917. 85409-1-PBS was HRP conjugated as the detection antibody. Range: 0.47-30 ng/mL

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  Sample test of MP01953-3

  The mean DNA ligase III/LIG3 concentration was determined to be 6.31 ng/mL in HeLa cell extract based on a 2.1 mg/mL extract load and 10.39 ng/mL in HEK-293T cell extract based on a 2.8 mg/mL extract load.

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  Affinity and Kinetic Characterization of 85409-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 85409-1-PBS against Human DNA ligase III/LIG3 were performed. The affinity constant is 1.18 nM.

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Geprüfte Anwendungen

Produktinformation

85409-1-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test DNA ligase III/LIG3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: DNA ligase III/LIG3 fusion protein Ag24917
• Vollständiger Name: ligase III, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC068005
• Gene symbol: LIG3
• Gene ID (NCBI): 3980
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.