LIG4 Polyklonaler Antikörper

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LIG4 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12695-1-PBS

Synonyme

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  WB analysis of mouse testis using 12695-1-AP (same clone as 12695-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12695-1-AP (same clone as 12695-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 12695-1-AP (same clone as 12695-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12695-1-AP (same clone as 12695-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 12695-1-AP (same clone as 12695-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 12695-1-AP (LIG4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12695-1-AP (LIG4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12695-1-AP (same clone as 12695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12695-1-AP (LIG4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12695-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12695-1-PBS bindet in WB, IHC, Indirect ELISA LIG4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIG4 fusion protein Ag3385
• Vollständiger Name: ligase IV, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 911 aa, 104 kDa
• Beobachtetes Molekulargewicht: 100-104 kDa
• GenBank-Zugangsnummer: BC037491
• Gene symbol: LIG4
• Gene ID (NCBI): 3981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.