LIG4 Monoklonaler Antikörper

LIG4 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H6C11

Kat-Nr. : 66705-1-PBS

Synonyme

DNA ligase IV, DNA ligase 4, EC:6.5.1.1, LIG 4, Ligase IV

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  WB analysis of PC-3 using 66705-1-Ig (same clone as 66705-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66705-1-Ig (same clone as 66705-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66705-1-Ig (same clone as 66705-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of Ramos using 66705-1-Ig (same clone as 66705-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66705-1-Ig (same clone as 66705-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66705-1-Ig (LIG4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66705-1-Ig (same clone as 66705-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66705-1-Ig (LIG4 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66705-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66705-1-PBS bindet in WB, IF/ICC, Indirect ELISA LIG4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LIG4 fusion protein Ag3385
• Vollständiger Name: ligase IV, DNA, ATP-dependent
• Berechnetes Molekulargewicht: 911 aa, 104 kDa
• Beobachtetes Molekulargewicht: 100-104 kDa
• GenBank-Zugangsnummer: BC037491
• Gene symbol: LIG4
• Gene ID (NCBI): 3981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand brdownloadeaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.