LIGHT/CD258 Rekombinanter Antikörper

LIGHT/CD258 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240694D7

Kat-Nr. : 98063-1-RR

Synonyme

LIGHT, HVEM-L, HVEML, HVEM L, CD258

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  FC experiment of human PBMCs using 98063-1-RR

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD8a. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98063-1-RR

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated (red) human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98063-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.25 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA, Ionomycin and Brefeldin A treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98063-1-RR bindet in FC LIGHT/CD258 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 14
• Berechnetes Molekulargewicht: 26KD
• GenBank-Zugangsnummer: NM_003807
• Gene symbol: LIGHT
• Gene ID (NCBI): 8740
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.