LIGHT/CD258 Rekombinanter Antikörper

LIGHT/CD258 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240694D7

Kat-Nr. : CL594-98063

Synonyme

LIGHT, 240694D7, CD258, HVEM L, HVEML

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  FC experiment of human PBMCs using CL594-98063

  1x10^6 untreated or PMA, ionomycin and Brefeldin A treated human PBMCs were surface stained with APC Anti-Human CD8a and 5 ul CoraLite®594 Anti-Human LIGHT/CD258 Rabbit RecAb (CL594-98063, Clone: 240694D7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98063

  1x10^6 PMA, ionomycin and Brefeldin A treated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human LIGHT/CD258 Rabbit RecAb (CL594-98063, Clone: 240694D7) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human LIGHT/CD258 Rabbit RecAb (CL594-98063, Clone: 240694D7) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98063 bindet in FC LIGHT/CD258 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LIGHT/CD258 fusion protein Eg32095
• Vollständiger Name: tumor necrosis factor (ligand) superfamily, member 14
• Berechnetes Molekulargewicht: 26KD
• GenBank-Zugangsnummer: NM_003807
• Gene symbol: LIGHT
• Gene ID (NCBI): 8740
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.