LILRB3/CD85a Rekombinanter Antikörper

LILRB3/CD85a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241894C10

Kat-Nr. : 98237-1-PBS

Synonyme

LILRB3, ILT-5, ILT5, HL9, CD85a

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  FC experiment of human PBMCs using 98237-1-RR (same clone as 98237-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB3/CD85a Rabbit Recombinant Antibody (98237-1-RR, Clone: 241894C10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98237-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98237-1-RR (same clone as 98237-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB3/CD85a Rabbit Recombinant Antibody (98237-1-RR, Clone: 241894C10)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98237-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98237-1-PBS bindet in FC LILRB3/CD85a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3
• Berechnetes Molekulargewicht: 631 aa, 69 kDa
• GenBank-Zugangsnummer: BC112198
• Gene symbol: LILRB3
• Gene ID (NCBI): 11025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

The leukocyte immunoglobulin-like receptors (LIRs, also known as ILTs, CD85, and LILRs) comprise a family of related immunoregulatory receptors encoded within the leukocyte receptor cluster (LRC) at chromosomal region 19q13.4 (PMID: 11491530). LIRs are transmembrane proteins containing either two or four extracellular immunoglobulin domains, and have diverse functions, including the regulation of inflammation, immune tolerance, cell differentiation and nervous system plasticity (PMID: 16406677; 26040207). LILRB3, also known as ILT-5 or CD85a, belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB3 is found on the surface of a variety of cell types including monocytes/macrophages, granulocytes, NK cells and some T cells. It binds to MHC class I molecules and transduces a negative signal that inhibits stimulation of an immune response. LILRB3 has been reported as a myeloid cell checkpoint that elicits profound immunomodulation (PMID: 32870822).