LILRB3/CD85a Rekombinanter Antikörper

LILRB3/CD85a Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241894C10

Kat-Nr. : 98237-1-RR

Synonyme

LILRB3, ILT-5, ILT5, HL9, CD85a

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  FC experiment of human PBMCs using 98237-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB3/CD85a Rabbit Recombinant Antibody (98237-1-RR, Clone: 241894C10) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98237-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRB3/CD85a Rabbit Recombinant Antibody (98237-1-RR, Clone: 241894C10)(red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98237-1-RR bindet in FC LILRB3/CD85a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 3
• Berechnetes Molekulargewicht: 631 aa, 69 kDa
• GenBank-Zugangsnummer: BC112198
• Gene symbol: LILRB3
• Gene ID (NCBI): 11025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The leukocyte immunoglobulin-like receptors (LIRs, also known as ILTs, CD85, and LILRs) comprise a family of related immunoregulatory receptors encoded within the leukocyte receptor cluster (LRC) at chromosomal region 19q13.4 (PMID: 11491530). LIRs are transmembrane proteins containing either two or four extracellular immunoglobulin domains, and have diverse functions, including the regulation of inflammation, immune tolerance, cell differentiation and nervous system plasticity (PMID: 16406677; 26040207). LILRB3, also known as ILT-5 or CD85a, belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). LILRB3 is found on the surface of a variety of cell types including monocytes/macrophages, granulocytes, NK cells and some T cells. It binds to MHC class I molecules and transduces a negative signal that inhibits stimulation of an immune response. LILRB3 has been reported as a myeloid cell checkpoint that elicits profound immunomodulation (PMID: 32870822).