ILT-3/LILRB4 Monoklonaler Antikörper

ILT-3/LILRB4 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8E1

Kat-Nr. : 67119-1-Ig

Synonyme

LILRB4, Immunoglobulin-like transcript 3, ILT-3, ILT3, CD85k

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  WB analysis of MCF-7 using 67119-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 67119-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67119-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67119-1-Ig (LILRB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 67119-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using LILRB4 antibody (67119-1-Ig, Clone: 1C8E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HUVEC-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67119-1-Ig bindet in WB, IF/ICC, ELISA ILT-3/LILRB4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ILT-3/LILRB4 fusion protein Ag3219
• Vollständiger Name: leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4
• Berechnetes Molekulargewicht: 448 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC026309
• Gene symbol: LILRB4
• Gene ID (NCBI): 11006
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.