LIN54 Polyklonaler Antikörper

LIN54 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 26644-1-AP

Synonyme

CXCDC1, JC8.6, KIAA2037, lin 54 homolog (C. elegans), LIN54, MIP120, Protein lin 54 homolog

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  WB analysis using 26644-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26644-1-AP (LIN54 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26644-1-AP bindet in WB, ELISA LIN54 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIN54 fusion protein Ag24507
• Vollständiger Name: lin-54 homolog (C. elegans)
• Berechnetes Molekulargewicht: 749 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa, 100 kDa
• GenBank-Zugangsnummer: BC109278
• Gene symbol: LIN54
• Gene ID (NCBI): 132660
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN54 is a component of the LINC/DREAM complex, an important regulator of cell cycle genes (PMID: 17671431; 19725879). The complex binds to more than 800 promoters in G0 phase and is required for repression of E2F target genes (PMID:17531812). In S phase, the complex binds to the promoters of G2/M genes whose products are required for mitosis and plays an important role in their cell cycle dependent activation (PMID:17671431). LIN54 has a DNA binding region (CXC-hinge-CXC, CHC domain), which consists of two cysteine-rich (CXC) domains separated by a short spacer. It has been reported that the CHC domain functions as a DNA binding domain, and conserved cysteine residues in two CXC domains are essential for DNA binding (PMID: 22895175). The calculated molecular weight of LIN54 is 79 kDa and an apparent molecular mass of 100-110 kDa has been reported (PMID: 17531812; 28920576; 17671431).

Publikationen

Species	Application	Title
	WB	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma