LIN9 Polyklonaler Antikörper

LIN9 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 17882-1-AP

Synonyme

TGS, BARA, Beta subunit-associated regulator of apoptosis, hLin-9, HuLin-9

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  WB analysis using 17882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17882-1-AP (LIN9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17882-1-AP

  IP result of anti-LIN9 (IP:17882-1-AP, 4ug; Detection:17882-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, Maushodengewebe, NIH/3T3-Zellen, Rattenhodengewebe
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

17882-1-AP bindet in WB, IF, IP, CoIP, ELISA LIN9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIN9 fusion protein Ag11889
• Vollständiger Name: lin-9 homolog (C. elegans)
• Berechnetes Molekulargewicht: 558 aa, 64 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC045625
• Gene symbol: LIN9
• Gene ID (NCBI): 286826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.

Publikationen

Species	Application	Title
		Cell Death Dis; Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis; Authors - Yunshang Chen
human	WB,IF,CoIP	Int J Oncol; Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9; Authors - Zhenwei Ma
human	WB	Sci China Life Sci; PMID: 31420851; Authors - Hongna Lai
human	WB	EMBO Mol Med; Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer; Authors - Laura Pascual-Reguant
mouse	WB	FEBS Open Bio; The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells; Authors - Jie Tian
human	WB	J Transl Med; Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation; Authors - Qinghui Kan ; KD Validated