HSL Rekombinanter Antikörper

HSL Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241875B11

Kat-Nr. : 84540-1-PBS

Synonyme

LIPE, LHS, Hormone-sensitive lipase, EC:3.1.1.79, EC:3.1.1.23

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  WB analysis of human testis using 84540-1-RR (same clone as 84540-1-PBS)

  Human testis tissue were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  WB analysis using 84540-1-RR (same clone as 84540-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84540-1-RR (HSL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01374-1

  Sandwich ELISA standard curve of MP01374-1, Human HSL Recombinant Matched Antibody Pair - PBS only. 84540-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag11360. 84540-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01374-1

  The mean HSL concentration was determined to be 3.58 ng/mL in 3T3-L1 cell extract based on a 1.30 mg/mL extract load and 1.80 ng/mL in NIH/3T3 cell extract based on a 5.90 mg/mL extract load.

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  IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84540-1-RR (LIPE antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brown adipose using 84540-1-RR (same clone as 84540-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 84540-1-RR (HSL antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84540-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01374-1

  Cytometric bead array standard curve of MP01374-1, HSL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84540-3-PBS. Detection antibody: 84540-1-PBS. Standard: Ag11360. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84540-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84540-1-RR against Human HSL were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84540-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test HSL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HSL fusion protein Ag11360
• Vollständiger Name: lipase, hormone-sensitive
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC070041
• Gene symbol: HSL
• Gene ID (NCBI): 3991
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hormone sensitive lipase (HSL) belongs to the 'GDXG' lipolytic enzyme family. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. HSL has two isoforms with the molecular mass of 117 kDa and 84 kDa.