LIPF Polyklonaler Antikörper

LIPF Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25889-1-AP

Synonyme

lipase, gastric, HLAL, HGL, GL, Gastric lipase

---

• 

  WB analysis of MKN-45 using 25889-1-AP

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25889-1-AP (LIPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 25889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25889-1-AP (LIPF Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MKN-45-Zellen, Maus-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25889-1-AP bindet in WB, IHC, ELISA LIPF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIPF fusion protein Ag23149
• Vollständiger Name: lipase, gastric
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC112272
• Gene symbol: LIPF
• Gene ID (NCBI): 8513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

gastric lipase, is an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions.