LIPH Polyklonaler Antikörper

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• KD/KO Validated

LIPH Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16602-1-AP

Synonyme

LPDLR, LPD lipase-related protein, lipase, member H, Lipase member H, EC:3.1.1.-

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  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16602-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16602-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16602-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16602-1-AP (LIPH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 16602-1-AP

  IP result of anti-LIPH (IP:16602-1-AP, 4ug; Detection:16602-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 16602-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 16602-1-AP (LIPH antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 16602-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16602-1-AP (LIPH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, Mauslungengewebe
• Erfolgreiche IP: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• ELISA: See 1 publications below

Produktinformation

16602-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LIPH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIPH fusion protein Ag9775
• Vollständiger Name: lipase, member H
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC064941
• Gene symbol: LIPH
• Gene ID (NCBI): 200879
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIPH(Lipase member H) is also named as LPDLR, MPAPLA1, PLA1B. It is a phosphatidic acid-selective phospholipase A1 (PLA1) that produces 2-acyl lysophosphatidic acid (LPA) and belongs to the AB hydrolase superfamily and Lipase family.LIPH has a 12-residue lid region, which likely covers a catalytic pocket, and 4 potential N-linked glycosylation sites. Defects in LIPH are the cause of hypotrichosis type 7 (HYPT7).

Publikationen

Species	Application	Title
human	WB	Genet Med; Loss-of-function variants in C3ORF52 result in localized autosomal recessive hypotrichosis.; Authors - Liron Malki
human	IHC	Cell Oncol (Dordr); Genomic analysis and filtration of novel prognostic biomarkers based on metabolic and immune subtypes in pancreatic cancer; Authors - Guangyu Chen
human	WB	J Cell Mol Med; LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer.; Authors - Yixiao Zhang ; KD Validated
human	IHC	Tumour Biol; Lipase member H is a novel secreted protein associated with a poor prognosis for breast cancer patients.; Authors - Meizi Cui
human	ELISA,WB,IHC	Biochem Biophys Res Commun; Lipase member H is a novel secreted protein selectively upregulated in human lung adenocarcinomas and bronchioloalveolar carcinomas.; Authors - Yasuhiro Seki
human	IHC	Tumour Biol; Lipase member H frequently overexpressed in human esophageal adenocarcinomas.; Authors - Hisako Ishimine