LIS1 Polyklonaler Antikörper

LIS1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20678-1-PBS

Synonyme

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  WB analysis using 20678-1-AP (same clone as 20678-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20678-1-PBS bindet in WB, IHC, Indirect ELISA LIS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: NM_000430
• Gene symbol: LIS1
• Gene ID (NCBI): 5048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)