LITAF Polyklonaler Antikörper

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LITAF Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16797-1-PBS

Synonyme

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  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16797-1-AP (LITAF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 16797-1-AP (same clone as 16797-1-PBS)

  IP result of anti-LITAF (IP:16797-1-AP, 4ug; Detection:16797-1-AP 1:600) with HeLa cells lysate 1520ug. This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 16797-1-AP (same clone as 16797-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 16797-1-AP (LITAF antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 16797-1-AP (same clone as 16797-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16797-1-AP (LITAF antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16797-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16797-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA LITAF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LITAF fusion protein Ag10269
• Vollständiger Name: lipopolysaccharide-induced TNF factor
• Berechnetes Molekulargewicht: 161 aa, 17 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC008309
• Gene symbol: LITAF
• Gene ID (NCBI): 9516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lipopolysaccharide-induced TNF factor(LITAF) is a transcription factor that identified as a regulator of TNF-alpha gene expression. It may has a role in protein degradation pathways and in tumor necrosis factor alpha(TNF-alpha) gene expression. Also it may regulate the expression of the CCL2/MCP-1 chemokine through NFKB1.Defects in LITAF may be involved in extramammary Paget disease (EMPD) carcinogenesis