LIV-1/ZIP6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LIV-1/ZIP6 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14236-1-PBS

Synonyme

LIV 1, SLC39A6, ZIP6, Estrogen-regulated protein LIV-1, LIV1

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  WB analysis of HeLa using 14236-1-AP (same clone as 14236-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14236-1-AP (same clone as 14236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14236-1-AP (same clone as 14236-1-PBS)

  WB result of LIV-1/ZIP6 antibody (14236-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIV-1/ZIP6 transfected HeLa cells. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 14236-1-AP (same clone as 14236-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14236-1-AP (same clone as 14236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 14236-1-AP (same clone as 14236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14236-1-AP (LIV-1/ZIP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14236-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14236-1-PBS bindet in WB, IHC, Indirect ELISA LIV-1/ZIP6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LIV-1/ZIP6 fusion protein Ag5478
• Vollständiger Name: solute carrier family 39 (zinc transporter), member 6
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa, 48 and 32 kDa
• GenBank-Zugangsnummer: BC039498
• Gene symbol: SLC39A6/ZIP6
• Gene ID (NCBI): 25800
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The zinc transporter LIV-1 (also known as SLC39A6 or ZIP6) was originally identified as an estrogen-induced gene in breast cancer cells. Elevated LIV-1 expression is reportedly related to cancer progression in various types of cancer, including breast, prostate, pancreatic, cervical and liver cancers. While correlation of LIV-1 and prognosis may vary. LIV 1 has 3 isoforms with MW of 85-100 (glycosylation and phosphorylation), 48 and 32 kDa while migrates as different bands in different lysates in SDS-PAGE.