ERGIC-53 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ERGIC-53 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (2)

Kat-Nr. : 13364-1-AP

Synonyme

ERGIC 53, ERGIC-53, F5F8D, FMFD1, gp58, Lectin mannose binding 1, lectin, mannose binding, 1, LMAN1, MCFD1, MR60, Protein ERGIC 53

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Galerie der Validierungsdaten

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  WB analysis using 13364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13364-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13364-1-AP (ERGIC-53 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13364-1-AP

  IP result of anti-ERGIC-53 (IP:13364-1-AP, 4ug; Detection:13364-1-AP 1:400) with HepG2 cells lysate 1000 ug.

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  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13364-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ERGIC-53 antibody (13364-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A549 using 13364-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 13364-1-AP (ERGIC-53 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Jurkat-Zellen, Mausherzgewebe, Maushirngewebe, Mausmilzgewebe, MCF-7-Zellen, Rattenherzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Produktinformation

13364-1-AP bindet in WB, IP, IHC, IF, ELISA ERGIC-53 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ERGIC-53 fusion protein Ag4183
• Vollständiger Name: lectin, mannose-binding, 1
• Berechnetes Molekulargewicht: 510 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC032330
• Gene symbol: LMAN1
• Gene ID (NCBI): 3998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ERGIC-53 (also known as LMAN1 or MR60) is a membrane mannose-specific lectin that selectively transports its cargo proteins from ER to ER-Golgi intermediate compartment (ERGIC) and Golgi, functioning as a cargo transport receptor for glycoproteins (PMID: 24664723; 10559958). Mutations in ERGIC-53 cause combined deficiency of coagulation factors V and VIII (PMID: 9546392).

Publikationen

Species	Application	Title
human	WB	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang
human	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang
human	IF	Nat Commun; ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling; Authors - Maximilian Hirschenberger
mouse	IF	Nat Commun; Homeostatic regulation of STING by retrograde membrane traffic to the ER.; Authors - Kojiro Mukai
human	WB	Nat Commun; Improved secretion of glycoproteins using an N-glycan-restricted passport sequence tag recognized by cargo receptor.; Authors - Hirokazu Yagi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

pitna (Verified Customer) (05-26-2021)

for Ergic Immunoprecipitation validation for human brain

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: human brain

ERGIC-53 Antibody Western Blot,Immunoprecipitation validation (1:500 dilution) in human brain (Cat no:13364-1-AP)

FH

Boyan (Verified Customer) (02-19-2020)

For WB, this antibody could detect endogenous Ergic53 with a very clean band.

• Applications: Western Blot,