LMCD1 Monoklonaler Antikörper

LMCD1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A7B3

Kat-Nr. : 67834-1-PBS

Synonyme

Dyxin, LMCD1

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  WB analysis of U2OS using 67834-1-Ig (same clone as 67834-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 67834-1-Ig (same clone as 67834-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67834-1-Ig (LMCD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67834-1-Ig (LMCD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67834-1-Ig (same clone as 67834-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using LMCD1 antibody (67834-1-Ig, Clone: 2A7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67834-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67834-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LMCD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LMCD1 fusion protein Ag28331
• Vollständiger Name: LIM and cysteine-rich domains 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: ~40 kDa
• GenBank-Zugangsnummer: BC000646
• Gene symbol: LMCD1
• Gene ID (NCBI): 29995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LIM and cysteine-rich domains-1 (LMCD1) is a member of the LIM protein family, which contains an N-terminal cysteine-rich region, two C-terminal LIM domains and a central PET (Prickle, Espinas, and Testin) domain. LMCD1 has been reported in cardiac tissues and lung acting as a transcriptional repressor for GATA6. The mutations of LMCD1 promote cell migration and tumor metastasis in hepatocellular carcinoma. (PMID: 31501411) It has calculated molecular weight around 41kDa.