LMO7 Rekombinanter Antikörper

LMO7 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241781D11

Kat-Nr. : 84495-4-PBS

Synonyme

241781D11, FBX20, LOMP

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  WB analysis using 84495-4-RR (same clone as 84495-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84495-4-RR (LMO7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84495-4-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01336-4

  Sandwich ELISA standard curve of MP01336-4, Human LMO7 Recombinant Matched Antibody Pair - PBS only. 84495-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30016. 84495-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

• 

  Sample test of MP01336-4

  The mean LMO7 concentration was determined to be 70.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load and 99.53 ng/mL in mouse lung tissue extract based on a 1.6 mg/mL extract load.

• 

  Affinity and Kinetic Characterization of 84495-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 84495-4-PBS against Human LMO7 was performed. The affinity constant is 0.451 nM.

• 

  Affinity and Kinetic Characterization of 84495-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84495-4-RR against Human LMO7 were performed. The affinity constant is 0.246 nM.

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Geprüfte Anwendungen

Produktinformation

84495-4-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test LMO7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LMO7 fusion protein Ag30016
• Vollständiger Name: LIM domain 7
• Berechnetes Molekulargewicht: 193 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 180 kDa
• GenBank-Zugangsnummer: NM_005358
• Gene symbol: LMO7
• Gene ID (NCBI): 4008
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).