LMTK3 Polyklonaler Antikörper

LMTK3 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22899-1-PBS

Synonyme

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  WB analysis using 22899-1-AP (same clone as 22899-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22899-1-AP (LMTK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22899-1-AP (same clone as 22899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 22899-1-AP (same clone as 22899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22899-1-AP (LMTK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22899-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22899-1-PBS bindet in WB, IHC, Indirect ELISA LMTK3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: lemur tyrosine kinase 3
• Berechnetes Molekulargewicht: 154 kDa
• Beobachtetes Molekulargewicht: ~155 kDa
• GenBank-Zugangsnummer: NM_001080434
• Gene symbol: LMTK3
• Gene ID (NCBI): 114783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LMTK3 is enriched at a perinuclear structure around Golgi membranes, it is present as a dotted pattern throughout neurites, and localized at neuronal synapses. It is also expected to be expressed in brain and duodenum. LMTK3 is a serine/threonine protein kinase expressed predominantly in brain. LMTK3 regulated ER-alpha stability and activity directly at the mRNA level via downregulation of PKC catalytic activity, resulting in less phosphorylated AKT at ser473. In vitro kinase assays indicated that LMTK3 inhibited the ability of PKC to phosphorylate ER-alpha, thereby affecting ER-alpha activity indirectly at the protein level by protecting it from proteasomal degradation. In agreement, analysis with a breast tumor xenograft mouse model revealed that knockdown of LMTK3 reduced proliferation of ER-alpha-positive breast cancer cells and reduced tumor growth. In addition, LMTK3 abundance and intronic polymorphisms were significantly associated with human cancers, overall patient survival, and predicted response to endocrine therapies. The calculated molecular weight of LMTK3 is 153 kDa.