LNPEP Polyklonaler Antikörper

LNPEP Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55154-1-PBS

Synonyme

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  WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 55154-1-AP (same clone as 55154-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55154-1-PBS bindet in WB, IHC, Indirect ELISA LNPEP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: leucyl/cystinyl aminopeptidase
• Berechnetes Molekulargewicht: 117 kDa
• Beobachtetes Molekulargewicht: 117-125 kDa
• GenBank-Zugangsnummer: NM_005575
• Gene symbol: LNPEP
• Gene ID (NCBI): 4012
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.